Docking of human rhodopsin mutant (Gly90àAsp) with beta-arrestin and cyanidin 3-rutinoside to cure night blindness

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Docking of human rhodopsin mutant (Gly90→Asp) with beta-arrestin and cyanidin 3-rutinoside to cure night blindness

MOTIVATION Rhodopsin is a visual pigment present in rod cells of retina. It belongs to GPCR family and involves photoisomerization of 11-cis-retinal to all-trans-retinal isomers, conformational changes in rhodopsin and signal transduction cascade to generate a nerve impulse. This signaling pathway has been targeted to eliminate the effect of a mutation (Gly90→Asp) responsible for abnormal activ...

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Vision Science: Can Rhodopsin Cure Blindness?

Outer retinal degeneration is the leading cause of blindness in the developed world. A new study now demonstrates that ectopic expression of human rhodopsin in the inner retina, mediated by viral gene therapy, can restore light sensitivity and some vision to mice blind from outer retinal degeneration.

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Night Blindness and Its Speedy Cure with Liver

Sir,?In your August number, I have just read Dr. J. M. Richardson's letter regarding night-blindness and its speedy cure under treatment with liver. Obviously, there are cases of functional night-blindness in which there are no ophthalmic changes and which are the result merely of defective nutrition, anaemia, ill-health, etc. The rapid improvement under liver treatment is not, therefore, surpr...

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Night blindness and the mechanism of constitutive signaling of mutant G90D rhodopsin.

The G90D rhodopsin mutation is known to produce congenital night blindness in humans. This mutation produces a similar condition in mice, because rods of animals heterozygous (D+) or homozygous (D+/+) for this mutation have decreased dark current and sensitivity, reduced Ca(2+), and accelerated values of tau(REC) and tau(D), similar to light-adapted wild-type (WT) rods. Our experiments indicate...

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Dynamics of arrestin-rhodopsin interactions: acidic phospholipids enable binding of arrestin to purified rhodopsin in detergent.

We report that acidic phospholipids can restore the binding of visual arrestin to purified rhodopsin solubilized in n-dodecyl-beta-d-maltopyranoside. We used this finding to investigate the interplay between arrestin binding and the status of the retinal chromophore ligand in the receptor binding pocket. Our results showed that arrestin can interact with the late photoproduct Meta III and conve...

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ژورنال

عنوان ژورنال: Bioinformation

سال: 2012

ISSN: 0973-8894,0973-2063

DOI: 10.6026/97320630008128